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. Author manuscript; available in PMC: 2022 Oct 6.
Published in final edited form as: Neuron. 2021 Aug 13;109(19):3104–3118.e6. doi: 10.1016/j.neuron.2021.07.018

Fig. 6: Rnf43 regulates OPC Fzd1 surface expression.

Fig. 6:

(A) Quantification of western blots for Fzd1-10 protein levels in surface membrane extractions from Olig1cre:RZ fl/fl and RZ fl/fl control OPCs (n=4 independent experiments). Na-K-ATPase was used as loading control. (B) Western blot for Fzd1, Fzd2, Fzd6 and Fzd9 (n=4 independent experiments) in Olig1cre:RZ fl/fl and RZ fl/fl control OPC membranes. (C) Immunostaining for HA and GFP tags (and DAPI) in hela cells transfected with the following vectors: left, FZD1-HA and empty control vector-GFP; right, FZD1-HA and Rnf43-GFP. The location of FZD1 is indicated by HA tag localization. (D) Quantification from the above vector transfections in (C) of Fzd1 localization on cell membrane or in cytoplasm by quantification of HA+ foci (n=3 independent experiments). (E) FZD1 (red) expression in NG2+ OPCs (green) in vitro following isolation from Olig1cre:RZ fl/fl and RZ fl/fl control mice (E), and in vivo in 21dpl remyelinating corpus callosum lesions from these same mice. Values in graphs are mean±s.d. NS not significant, *p<0.05, **** p<0.0001. Scale bars are 20μm (in c, e) and 10μm in (f). See also Fig. S7.