KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat anti-PDGFRα | BD Biosciences | Cat# 558774, RRID:AB_397117 |
| Rabbit anti-PDGFRα | Gift from W. Stallcup | Kucharova et al., 2011 |
| Rabbit anti-Olig2 | Gift from C.D. Stiles | Arnett et al., 2014 |
| Rabbit anti-RNF43 | Abcam | Cat# ab84125, RRID:AB_2181252 |
| Rabbit anti-NF200 | Sigma-Aldrich | Cat# N4142, RRID:AB_477272 |
| Rabbit anti-Rnf43 | Abcam | Cat# ab217787 |
| Rat anti-FZD1 | R&D | Cat# MAB11201 |
| Rat anti-MBP | Serotec | Cat# MCA409S |
| Mouse anti-NG2 | Millipore | Cat# 05-710, RRID:AB_11214068 |
| Rabbit anti-Ki67 | Thermo Fisher scientific | Cat# MA5-14520, RRID:AB_10979488 |
| Mouse anti-Olig2 | Millipore | Cat# MABN50, RRID:AB_10807410 |
| Mouse anti-HA | Beyotime | Cat# AH158 |
| Goat anti-FZD2 | Invitrogen | Cat# PA5-47119 |
| Goat anti-FZD3 | R&D | Cat# AF1001 |
| Rat anti-FZD4 | R&D | Cat# MAB194 |
| Rabbit anti-FZD5 | Abcam | Cat# ab75234, RRID:AB_1523637 |
| Mouse anti-FZD6 | Santa Cruz | Cat# sc-393791, RRID:AB_2736833 |
| Rabbit anti-FZD7 | Novus Biologicals | Cat# NBP2-23624 |
| Rabbit anti-FZD8 | Novus Biologicals | Cat# NLS4767, RRID:AB_2109536 |
| Goat anti-FZD9 | R&D | Cat# AF2440 |
| Rabbit anti-FZD10 | Abcam | Cat# ab83044, RRID:AB_1860570 |
| Rabbit anti-Na-K-ATPase | Cell signaling technology | Cat# 3010, RRID:AB_2060983 |
| Rabbit Anti-Rnf43 | Invitrogen | Cat# PA5-79930 |
| Normal Rabbit IgG | Cell signaling technology | Cat# 2729S |
| Sheep anti-Digoxigenin-AP Fab fragments | Sigma-Aldrich | Cat# 11093274910 |
| Biological samples | ||
| Human MS Tissue | Imperial College London, MS Brain Bank, UK | http://www.imperial.ac.uk/medicine/multiple-sclerosis-and-parkinsons-tissue-bank |
| Human newborn HIE tissue and controls | UCSF Pediatric Neuropathology Research Laboratory (PNRL) | https://pediatrics.ucsf.edu/neonatology/newborn-brain-research-institute |
| Chemicals, peptides, and recombinant proteins | ||
| Tamoxifen | Sigma-Aldrich | Cat# T5648 |
| Lysolecithin | Sigma-Aldrich | Cat# L4129 |
| UM206 | ChinaPeptides | Laeremans et al., 2011 |
| Recombinant Murine Wnt3a | PeproTech | Cat# 315-20, |
| Human recombinant PDGF-AA | PeproTech | Cat# 100-13A |
| Critical commercial assays | ||
| NBT/BCIP Stock Solution | Sigma-Aldrich | Cat# 11681451001 |
| Amaxa Nucleofector Kit | Lonza | Cat# VPI-1006 |
| Mem-PER™ Plus Membrane Protein Extraction Kit | Thermo Fisher scientific | Cat# 89842 |
| ECL™ Prime Western blotting detection reagents | GE Healthcare | Cat# RPN2232 |
| RNeasy Plus Mini Kit | Qiagen | Cat# 74134 |
| GoTaq® qPCR Master Mix | Promega | Cat# A6001 |
| Protein A/G PLUS-Agarose | Santa Cruz | Cat# sc-2003, RRID:AB_10201400 |
| Experimental models: Cell lines | ||
| Hela cell line | ATCC | CCL-2 |
| Oli-neu cell line | Jung et al., 1995 | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: Rnf43 fl/fl | Koo et al., 2012 | N/A |
| Mouse: Znrf3 fl/fl | Koo et al., 2012 | N/A |
| Mouse: Olig1-cre | Arnett et al., 2004 | N/A |
| Mouse: Olig2-cre | Schuller et al., 2008 | N/A |
| Mouse: PDGFRa-creER | Kang et al., 2010 | N/A |
| Mouse: Rosa-YFP | Jackson laboratory | Cat# 006148 |
| Mouse: Aldh1L1-GFP | Jackson laboratory | Cat# 030248 |
| Mouse: Cx3cr1-GFP | Jackson laboratory | Cat# 032127 |
| Mouse: APC fl/fl | Robanus-Maandag et al., 2010 | Cat# 029275 |
| Oligonucleotides | ||
| Primer: Rnf43 Forward: CAGCCAGTGTGGTTGTATCT Reverse: ACCTGGCAATGTGAGTAAGG |
This paper | N/A |
| Primer: Znrf3 Forward: TCTCTTCTGTGCCCTCTTCT Reverse: GCTCCTTGTCCATGGCTATAC |
This paper | N/A |
| Primer: Axin2 Forward: CCAGGTGGACCAAGTCTTTAC Reverse: TCATCTGCCTGAACCCATTAC |
This paper | N/A |
| Primer: Notum Forward: AACGTGGCACAGTTCCTTAT Reverse: CACACCCTCTAGTTCCCATTAC |
This paper | N/A |
| Primer: Olig2 Forward: GGCGGTGGCTTCAAGTCATC Reverse: TCGGGCTCAGTCATCTGCTTC |
This paper | N/A |
| Primer: PDGFRα Forward: GGGGAGAGTGAAGTGAGCTG Reverse: CATCCGTCTGAGTGTGGTTG |
This paper | N/A |
| Primer: MBP Forward: AATGCGGAAAGGAAGGAGAG Reverse: TGTCCGATCAGGGCTAGTAT |
This paper | N/A |
| Primer: GAPDH Forward: AGGCCGGTGCTGAGTATGTC Reverse: TGCCTGCTTCACCACCTTCT |
This paper | N/A |
| Recombinant DNA | ||
| Plasmid: FZD1-HA | This paper | N/A |
| Plasmid: Rnf43-GFP | This paper | N/A |
| Software and algorithms | ||
| Image-Pro Plus software 5.0 | Media Cybernetics | https://www.mediacy.com/imageproplus; RRID: SCR_007369 |
| GraphPad Prism 6 | GraphPad Software | https://www.graphpad.com/scientificsoftware/prism/; RRID:SCR_015807 |
| ZEN Digital Imaging for Light Microscopy | Zeiss | RRID:SCR_013672 |
| Olympus Confocal Laser Scanning Microscope software | Olympus | RRID:SCR_017015 |