Antibodies |
α-RPS6-phospho-S240 (RPS6-P) |
Dobrenel et al., 2016b
|
N/A |
α-RPS6-Total |
Christian Meyer, Dobrenel et al., 2016b
|
N/A |
Goat anti-rabbit Immunoglobulin G (IgG) Horseradish Peroxidase (HRP) Conjugate |
Cell Signaling Technology |
Cat# 7074; RRID:AB_2099233 |
Chemicals, peptides, and recombinant proteins |
DCMU, 3-(3,4-dichlophenyl)-1,1-dimethylurea |
Sigma-Aldrich |
Cat# D2425; CAS# 330-54-1; PubChem Substance ID 57654085 |
DNP, 2,4-dinitrophenol |
Sigma-Aldrich |
Cat# D198501; CAS# 51-28-5; PubChem Substance ID 24893583 |
AZD-8055 |
Chemdea LLC |
Cat# CD0348 |
Torin2 |
Cayman Chemical |
Cat# 14185; CAS# 1223001-51-1 |
Deposited data |
Torin2 RNA-seq data |
NCBI SRA, Scarpin et al., 2020
|
Project PRJNA639161 |
Root RNA-seq data 4- and 7-days old seedlings |
Gene Expression Omnibus (GEO) database, Miotto et al., 2019
|
Accession number GEO: GSE132249
|
Acute light treatment |
Gene Expression Omnibus (GEO) database, Mancini et al., 2016
|
Accession number GEO: GSE68560
|
Western blots, RT-PCR and RT-qPCR data |
Mendeley |
https://doi.org/10.17632/xt8sznfw4s.1
|
Experimental models: organisms/strains |
Arabidopsis thaliana Col 0 |
Dr. M. Yanovsky (FIL, Buenos Aires, Argentina) |
N/A |
Tor RNAi line, RNAi line of TOR (TARGET OF RAPAMYCIN) 35-7 |
Dr. Christian Meyer, Deprost et al., 2007
|
N/A |
Oligonucleotides |
Primers for RT-PCR splicing analysis, see Table S6
|
This paper |
N/A |
Primers for RT-qPCR, see Table S6
|
This paper |
N/A |
Software and algorithms |
Infostat |
https://www.infostat.com.ar/
|
N/A |
3D RNA-seq App |
https://github.com/wyguo/ThreeDRNAseq; https://3drnaseq.hutton.ac.uk/app_direct/3DRNAseq/; Guo et al., 2020. |
N/A |
Galaxy - Salmon |
https://usegalaxy.org/; Salmon tool; Patro et al., 2017. |
N/A |
Boxify - Drawing isoforms and checking RT-PCR product sizes |
https://boxify.boku.ac.at/; this paper. |
N/A |