Table 2.
Binding kinetic values from surface biolayer interferometry
| Protein | SARS-CoV-2 spike |
|
pIgR |
|
|||
|---|---|---|---|---|---|---|---|
|
kon (min−1 nM−1) |
koff (min−1) |
Kd (nM) |
kon (min−1 nM−1) |
koff (min−1) |
Kd (nM) |
Relative Kd (VHH2) | |
| CV19B277 | 32157 | 0.0008426 | 26.2 | 39.9 | 0.008349 | 2 x 105 | 142857 |
| CV19B308 | 2975 | 0.003050 | 1.025 x 103 | 63861 | 8.646 x 10–5 | 1.4 | 1 |
| CV19B307 | 8646 | 0.001496 | 173.1 | 44364 | 0.0002236 | 5.0 | 4 |
| CV19B301 | 10783 | 0.001092 | 101.2 | 13406 | 0.003557 | 265.3 | 190 |
| CV19B290 | 37.88 | 0.008619 | 2.28 x 105 | NB | NB | NB | NB |
| CV19B289 | 15478 | 0.002270 | 146.7 | NB | NB | NB | NB |
| CV19B283 | 28303 | 0.0009639 | 34.1 | 5.1 x 104 | 0.0004055 | 7.8 | 6 |
| CV19B265 | 37364 | 0.001704 | 45.6 | NB | NB | NB | NB |
| D001 | 43749 | 0.0006574 | 15.0 | NB | NB | NB | NB |
| SAD-S35 | 37484 | 0.0005347 | 14.3 | NB | NB | NB | NB |