Figure 4.

Microbiota can partly induce a CR-like phenotype in the intestine. The mice were fed ad libitum, submitted CR, or treated with antibiotics followed by fecal bacteria transfer (FT ad lib and FT CR; A). Gene expression of the different GST subtypes (b-c), GST activity (d), as well as levels of GSH (e), were measured in the jejunal mucosa. The levels of GSH-taurine conjugate (f, i), free taurine (g, j), and taurine conjugate m/z 249 (h, k) were measured in the jejunum (f-h) and ileum (i-k). Statistical significance between CR and ad libitum groups was evaluated using two-tailed Student’s t-tests; n = 8; *p < .05. Data are presented as mean±SEM