Skip to main content
. 2021 Sep 15;10:e69508. doi: 10.7554/eLife.69508

Figure 2. H3K27me3 and H3K18ac are co-localized on camalexin biosynthesis genes in planta to form a bivalent chromatin.

Figure 2.

(A) Camalexin biosynthesis pathway map and the essential genes that can produce camalexin. (B-C) Sequential chromatin immunoprecipitation (ChIP)-qPCR confirms the co-localization of H3K27me3 and H3K18ac. Altering the order of antibodies against H3K27me3 or H3K18ac in two rounds of pull-downs shows similar results in sequential ChIP-qPCR. Negative control represents a genomic region that has low abundance of both H3K27me3 and H3K18ac. H3K27me3_only represents a genomic region that has high abundance of H3K27me3 and low abundance of H3K18ac. H3K18ac_only represents a genomic region that has high abundance of H3K18ac and low abundance of H3K27me3. NoAb represents no antibody in the second pull-down. Error bars represent standard deviation of data from six biological replicates. The experiments were performed twice with different plant samples and data were combined in this analysis.

Figure 2—source data 1. Source data used to generate Figure 2B,C.