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. 2021 Oct 26;10:e65580. doi: 10.7554/eLife.65580

Figure 7. nAChR-mediated modulation of synaptic transmission in CA2.

(A) Sample traces showing spontaneous excitatory postsynaptic currents (sEPSCs) of a CA2 pyramidal neuron recorded at ECl− in control (black) and in the presence of nicotine (1 μM; orange). (B) Aligned dot plots showing the mean frequency (left) and amplitude (right) of sEPSCs recorded from CA2 pyramidal neurons in control (black) and in the presence of nicotine (1 μM; orange) (n = 13 cells; frequency: control: 0.81 ± 0.1 Hz, Nic: 1.5 ± 0.2 Hz; p = 0.0005; amplitude: control: 19.9 ± 1.4 pA, Nic: 19.7 ± 0.8 pA; p = 0.76; Wilcoxon test). (C) Sample traces showing sEPSCs of a CA2 pyramidal neuron recorded at ECl− in the presence of α7 nAChR antagonist methyllycaconitine (MLA, (10 nM; black)) and in the presence of MLA plus nicotine (1 μM; orange). (D) Aligned dot plots showing the mean frequency (left) and amplitude (right) of sEPSCs recorded from CA2 pyramidal neurons in the presence of MLA (10 nM; black) and in the presence of MLA plus nicotine (1 μM; orange) (n = 9 cells; frequency: MLA: 1.9 ± 0.3 Hz, MLA+ Nic: 1.7 ± 0.2 Hz; p = 0.34; amplitude: MLA: 15.9 ± 1.1 pA, MLA+ Nic: 17.3 ± 1.0 pA; p = 0.36; Wilcoxon test). (E) Sample traces showing sEPSCs of a CA2 pyramidal neuron recorded at ECl− in the presence of non-α7 nAChR antagonist dihydro-β-erythroidine (DHβE, 0.5 μM; black) and in the presence of DHβE plus nicotine (1 μM; orange). (F) Aligned dot plots showing the mean frequency (left) and amplitude (right) of sEPSCs recorded from CA2 pyramidal neurons in the presence of DHβE (0.5 μM; black) or DHβE plus nicotine (1 μM; orange) (n = 9 cells; frequency: DHβE: 0.9 ± 0.1 Hz, DHβE + Nic: 1.05 ± 0.3 Hz; p > 0.99; amplitude: DHβE: 16.3 ± 1.5 pA, DHβE + Nic: 18.2 ± 1.2 pA; p = 0.25; Wilcoxon test). Open or closed circles represent values from single cells. Laterally located circles represent mean ± SEM. ***: p < 0.001.

Figure 7—source data 1. Frequency and amplitude of spontaneous excitatory postsynaptic currents .

Figure 7.

Figure 7—figure supplement 1. nAChR-mediated modulation of miniature synaptic events in CA2.

Figure 7—figure supplement 1.

(A) Sample traces showing miniature inhibitory and excitatory postsynaptic currents (mIPSCs) from a CA2 pyramidal neuron before (black) and in the presence of nicotine (Nic 1 µM; orange). (B) Aligned dot plots showing the mean frequency (left) and amplitude (right) of mIPSC recorded from CA2 pyramidal neurons in control (black) and in the presence of nicotine (1 µM; orange) (n = 7 cells; frequency: control: 1.5 ± 0.2 Hz, Nic: 1.2 ± 0.1 Hz; p = 0.03; amplitude: control: 19.2 ± 1.4 pA, Nic: 19.8 ± 0.9 pA; p = 0.69; Wilcoxon test). (C) Sample traces showing mEPSCs from a CA2 pyramidal neuron in control (black) and in the presence of nicotine (Nic 1 µM; orange). (D) Aligned dot plots showing the mean frequency (left) and amplitude (right) of mEPSCs recorded from CA2 pyramidal neurons in control (black) and in the presence of nicotine (Nic 1 µM; orange) (n = 8 cells; frequency: control: 0.37 ± 0.05 Hz, Nic: 0.32 ± 0.05 Hz; p = 0.72; amplitude: control: 16.4 ± 0.6 pA, Nic: 16.3 ± 1.3 pA; p = 0.69; Wilcoxon test). Open or closed circles represent values from single cells. Laterally located circles represent mean ± SEM. *: p < 0.05.
Figure 7—figure supplement 2. nAChR-mediated modulation of synaptic transmission in CA3 and CA1 regions.

Figure 7—figure supplement 2.

(A) Sample traces showing spontaneous excitatory postsynaptic currents (sEPSCs) of a CA3 pyramidal neuron in control (black) and in the presence of nicotine (1 µM; orange) recorded at ECl−. (B) Aligned dot plots showing the mean frequency (left) and amplitude (right) of sEPSCs recorded from CA3 pyramidal neurons in control (black) and in the presence of nicotine (1 µM; orange) (n = 9 cells; frequency: control: 5.3 ± 0.7 Hz, Nic: 4.1 ± 0.6 Hz; p = 0.004; amplitude: control: 27.1 ± 1.6 pA, Nic: 27.2 ± 2.2 pA; p = 0.9; Wilcoxon test). (C) Sample traces showing sEPSCs of a CA1 pyramidal neuron in control (black) and in the presence of nicotine (1 µM; orange) recorded at ECl−. (D) Aligned dot plots showing the mean frequency (left) and amplitude (right) of sEPSCs recorded from CA1 pyramidal neurons in control (black) and in the presence of nicotine (1 µM; orange) (n = 10 cells; frequency: control: 1.7 ± 0.5 Hz, Nic: 1.3 ± 0.4 Hz; p = 0.039; amplitude: control: 20.3 ± 1.3 pA, Nic: 19.4 ± 0.7 pA; p = 0.9; Wilcoxon test). Open or closed circles represent values from single cells. Laterally located circles represent mean ± SEM. *: p < 0.05; **: p < 0.01.