Figure 6. Quantitative real-time PCR (qRT-PCR) and Western blot analyses on wild-type (WT) and myopalladin knockout (MKO) mice at basal conditions and following transaortic constriction (TAC).
(A) qRT-PCR analysis for Mypn, Palld, and markers of cardiac remodeling on left ventricular RNA from WT and MKO male mice at basal conditions and 4 days (D) and 4 weeks (W) after TAC. B2m was used for normalization. Data are represented as mean ± standard error of the mean (SEM) (n = 3–8 per group performed in triplicate). *p < 0.05, **p < 0.01, **p < 0.001; two-way analysis of variance (ANOVA) with Bonferroni’s multiple comparison test. (B) Western blot analysis on left ventricular lysate from WT and MKO male mice at basal conditions and 4 days (D) and 4 weeks (W) after TAC. Representative blots are shown (n = 3–4 per group). (C) Densitometric analysis for proteins that were significantly altered in MKO mice. Normalization was performed to total protein content as assessed on TGX Stain-Free gels (Bio-Rad Laboratories). Data are represented as mean ± SEM (n = 3–4 per group). *p < 0.05, **p < 0.01, ***p < 0.001; two-way ANOVA with Bonferroni’s multiple comparison test.
Figure 6—figure supplement 1. Immunofluorescence stainings of left ventricular tissue from the heart of 4-month-old wild-type (WT) and myopalladin knockout (MKO) male mice for palladin (PALLD) and myopalladin (MYPN)-interacting proteins.
