| Strain, strain background (male Mus musculus) |
MYPN knockout (MKO) mice (in C57BL/6J background) |
Filomena et al., 2020
|
N/A |
|
| Strain, strain background (Mus musculus) |
C57BL/6J |
The Jackson Laboratories |
Cat# 000664RRID:IMSR_JAX:000664
|
|
| Strain, strain background (Escherichia coli) |
DH5α electrocompetent cells |
New England BioLabs |
Cat# C2989K |
|
| Strain, strain background (Escherichia coli) |
BL21-CodonPlus (DE3)-RIPL strain |
Agilent Technologies |
Cat# 230280 |
|
| Strain, strain background (Saccharomyces cerevisiae) |
Y2H Gold yeast strain |
Takara Bio |
Cat# 630498 |
|
| Strain, strain background (Saccharomyces cerevisiae) |
L40 yeast strain |
Takara Bio |
N/A |
|
| Antibody |
Anti-MYPN (rabbit polyclonal) |
Yamamoto et al., 2013
|
N/A |
WB (1:1000) |
| Antibody |
Anti-PALLD 621 (rabbit polyclonal) |
Pogue-Geile et al., 2006
|
Kindly provided by Prof. Carol Otey, University of North Carolina, Chapel Hill, NC, USA |
WB (1:500) IF (1:30) |
| Antibody |
Anti-NEBL (rabbit polyclonal) |
Mastrototaro et al., 2015
|
N/A |
WB (1:500) |
| Antibody |
Anti-ANKRD1/CARP (rabbit polyclonal) |
Miller et al., 2003
|
N/A |
WB (1:200) IF (1:20) |
| Antibody |
Anti-Cypher (rabbit polyclonal) |
Zhou et al., 2001
|
Kindly provided by Prof. Ju Chen, University of California San Diego, La Jolla, CA, USA |
WB (1:500) IF (1:50) |
| Antibody |
Anti-α-actinin (mouse monoclonal) |
Merck Life Science |
Cat# A7811RRID:AB_476766
|
WB (1:50000) IF (1:250) |
| Antibody |
Anti-desmin (rabbit polyclonal) |
Abcam |
Cat# Ab8592RRID:AB_306653
|
IF (1:80) |
| Antibody |
Anti-MKL1/MRTF-A (rabbit polyclonal) |
Merck Life Science |
Cat# AV37504RRID:AB_1853972
|
IF (1:30) |
| Antibody |
Anti-MKL1/MRTF-A (rabbit polyclonal) |
Immunological Sciences |
Cat# AB-84312RRID:AB_2892156
|
WB (1:500) |
| Antibody |
Anti-desmoplakin 1/2 (mouse monoclonal) |
Bio-Rad Laboratories |
Cat# 2722–5204RRID:AB_619950
|
WB (1:750) |
| Antibody |
Anti-α-E-catenin (mouse monoclonal) |
Santa Cruz Biotechnology |
Cat# sc-9988RRID:AB_626805
|
WB (1:1000) |
| Antibody |
Anti-β-catenin (rabbit monoclonal) |
Cell Signaling Technology |
Cat# 8480RRID:AB_11127855
|
WB (1:1000) |
| Antibody |
Anti-γ-catenin (rabbit polyclonal) |
Immunological Sciences |
AB-90215RRID:AB_2892157
|
WB (1:1000) |
| Antibody |
Anti-vinculin (mouse monoclonal) |
Merck Life Science |
Cat# V9264RRID:AB_10603627
|
WB (1:2000) |
| Antibody |
Anti-N-cadherin (mouse monoclonal) |
Cell Signaling Technology |
Cat# 4061RRID:AB_10694647
|
WB (1:1000) |
| Antibody |
Anti-SORBS2 (mouse monoclonal) |
Merck Life Science |
Cat# SAB4200183RRID:AB_10638778
|
WB (1:750) |
| Antibody |
Anti-connexin 43 (mouse monoclonal) |
Thermo Fisher Scientific |
Cat# 35–5000RRID:AB_87322
|
WB (1:400) |
| Antibody |
Anti-pAkt-Ser473 (rabbit monoclonal) |
Immunological Sciences |
MAB-94111RRID:AB_2892158
|
WB (1:500) |
| Antibody |
Anti-Akt (rabbit polyclonal) |
Cell Signaling Technology |
Cat# 9272RRID:AB_329827
|
WB (1:1000) |
| Antibody |
Anti-pErk1/2-Thr202/Tyr204 (rabbit polyclonal) |
Cell Signaling Technology |
Cat# 4370RRID:AB_2315112
|
WB (1:500) |
| Antibody |
Anti-Erk1/2 (mouse monoclonal) |
Santa Cruz Biotechnology |
Cat# sc-514302RRID:AB_2571739
|
WB (1:1000) |
| Antibody |
Anti-pP38-Tyr182 (mouse monoclonal) |
Santa Cruz Biotechnology |
Cat# sc-166182RRID:AB_2141746
|
WB (1:500) |
| Antibody |
Anti-P38α/β (mouse monoclonal) |
Santa Cruz Biotechnology |
Cat# sc-7972RRID:AB_628079
|
WB (1:500) |
| Antibody |
Anti-calcineurin B (rabbit polyclonal) |
Novus Biochemical |
Cat# NBP1-32720RRID:AB_2168483
|
WB (1:500) |
| Antibody |
Anti-calsequestrin - (mouse monoclonal) |
BD Trasduction Labs |
Cat# C16420
|
WB (1:5000) |
| Antibody |
Anti-SERCA2A (mouse monoclonal) |
Affinity Bio Reagent (ABR) |
Cat# ma3-919RRID:AB_325502
|
WB (1:1000) |
| Antibody |
Anti-pPLN-Thr16 (rabbit polyclonal) |
Badrilla |
Cat# 010–12RRID:AB_2617047
|
WB (1:500) |
| Antibody |
Anti-PLN (mouse monoclonal) |
Novus Biochemical |
Cat# NB300-582RRID:AB_10000946
|
WB (1:500) |
| Antibody |
Anti-RyR2 (mouse monoclonal) |
Affinity Bio Reagent (ABR) |
Cat# ma3-916RRID:AB_2183054
|
WB (1:1000) |
| Antibody |
Anti-pRyR-Ser2808 (rabbit polyclonal) |
Badrilla |
Cat# A010-30APRRID:AB_2617052
|
WB (1:500) |
| Antibody |
Anti-sodium calcium exchanger (NCX) (mouse monoclonal) |
Merck Life Science |
Cat# N216RRID:AB_260750
|
WB (1:1000) |
| Antibody |
Anti-CACNA1C (rabbit polyclonal) |
Abcam |
Cat# Ab58552RRID:AB_879800
|
WB (1:500) |
| Antibody |
Anti-CACNA1S (mouse monoclonal) |
Abcam |
Cat# Ab2862RRID:AB_2069567
|
WB (1:500) |
| Antibody |
Anti-phosphoserine/ threonine (rabbit polyclonal) |
ECM Biosciences |
Cat# PP2551RRID:AB_1184778
|
WB (1:500) |
| Antibody |
Anti-pTTN-Ser3991 (rabbit polyclonal) |
Kötter et al., 2013
|
N/A |
WB (1:500) |
| Antibody |
Anti-pTTN-Ser4080 (rabbit polyclonal) |
Kötter et al., 2013
|
N/A |
WB (1:500) |
| Antibody |
Anti-pTTN-Ser12742 (rabbit polyclonal) |
Kötter et al., 2013
|
N/A |
WB (1:500) |
| Antibody |
Anti-actin (goat polyclonal) |
Santa Cruz Biotechnology |
Cat# sc-1615RRID:AB_630835
|
WB (1:10000) |
| Antibody |
Goat anti-mouse IgG (H + L) secondary antibody Alexa Fluor 488-conjugated IgG |
Thermo Fisher Scientific |
Cat# A11029RRID:AB_138404
|
IF (1:500) |
| Antibody |
Goat anti-rabbit IgG (H + L) secondary antibody Alexa Fluor 488-conjugated IgG |
Thermo Fisher Scientific |
Cat# A11034RRID:AB_2576217
|
IF (1:500) |
| Antibody |
Goat anti-mouse IgG (H + L) Secondary antibody Alexa Fluor 568-conjugated conjugate |
Thermo Fisher Scientific |
Cat# A11031RRID:AB_144696
|
IF (1:500) |
| Antibody |
Goat anti-rabbit IgG (H + L) Secondary antibody Alexa Fluor 568-conjugated conjugate |
Thermo Fisher Scientific |
Cat# A11036RRID:AB_10563566
|
IF (1:500) |
| Antibody |
Goat anti-mouse IgG (H + L) Secondary antibody Alexa Fluor 647-conjugated conjugate |
Thermo Fisher Scientific |
Cat# A21236RRID:AB_2535805
|
IF (1:500) |
| Antibody |
Goat anti-rabbit IgG (H + L) Secondary antibody Alexa Fluor 647-conjugated conjugate |
Thermo Fisher Scientific |
Cat# A21245RRID:AB_2535813
|
IF (1:500) |
| Antibody |
Goat anti-rabbit IgG Horseradish Peroxidase (HRP) |
Thermo Fisher Scientific |
Cat# 31460RRID:AB_228341
|
WB (1:5000) |
| Antibody |
Goat anti-mouse IgG-HRP |
Thermo Fisher Scientific |
Cat# 31430RRID:AB_228307
|
WB (1:5000) |
| Antibody |
Donkey anti-goat IgG-HRP |
Santa Cruz Biotechnology |
Cat# sc-2020RRID:AB_631728
|
WB (1:2000) |
| Recombinant DNA reagent |
Modified pLexA vector |
Stenmark et al., 1995
|
N/A |
|
| Recombinant DNA reagent |
pGBKT7 DNA-BD vector |
Takara Bio |
Cat# 630443 |
|
| Recombinant DNA reagent |
pGADT7 AD vector |
Takara Bio |
Cat# 630442 |
|
| Recombinant DNA reagent |
Human skeletal muscle cDNA library in the pGAD10 vector |
Clontech Laboratories |
HL4010AB |
|
| Recombinant DNA reagent |
pET-3d vector |
Merck Life Science |
Cat# 69421 |
|
| Recombinant DNA reagent |
pETM-14 |
Dümmler et al., 2005
|
N/A |
|
| Recombinant DNA reagent |
pET-3d 6xHis human MYPN C-term |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pETM-14 human MYPN C-term |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pET-3d-6xHis human Titin Z4-Z5 |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pETM-14 human PALLD C-term |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pGBKT7 human Titin IgZ4-Z5 |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pGADT7 human MYPN full-length |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pGADT7 human MYPN Ig3-end |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pGADT7 human MYPN Ig5-end |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pGADT7 human MYPN Ig3-4 |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pGADT7 human MYPN Ig4-end |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pGADT7 human PALLD C-term |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pLexA-titin Z3-Z5 |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Recombinant DNA reagent |
pLexA-titin Z4-Z5 |
This paper |
N/A |
Cloning primers in Supplementary file 1
|
| Sequence-based reagent |
qRT-PCR primers |
This paper |
N/A |
Supplementary file 1
|
| Commercial assay or kit |
In-Fusion HD Cloning kit |
Takara Bio |
Cat# 639650 |
|
| Commercial assay or kit |
Monolith NT Protein Labeling Kit RED-NHS |
NanoTemper Technologies |
Cat# L001 |
|
| Commercial assay or kit |
DC Protein Assay Kit II |
Bio-Rad Laboratories |
Cat# 5000112 |
|
| Commercial assay or kit |
Frozen-EZ Yeast Transformation II kit |
Zymo Research |
Cat# T2001 |
|
| Chemical compound, drug |
Aureobasidin A |
Takara Bio |
Cat# 630499 |
|
| Chemical compound, drug |
X-α-Gal |
Takara Bio |
Cat# 630,463 |
|
| Chemical compound, drug |
HisTrap column |
GE Healthcare |
|
|
| Chemical compound, drug |
HiLoad 16/600 Superdex 75 pg column |
GE Healthcare |
Cat# 28989333 |
|
| Chemical compound, drug |
TRIzol Reagent |
Thermo Fisher Scientific |
Cat# 15596026 |
|
| Chemical compound, drug |
High Capacity cDNA Reverse Transcription kit |
Thermo Fisher Scientific |
Cat# 4368814 |
|
| Chemical compound, drug |
GoTaq qPCR Master Mix |
Promega |
Cat# A6002 |
|
| Chemical compound, drug |
COmplete Protease Inhibitor Cocktail Tablets |
Merck Life Science |
Cat# 11697498001 |
|
| Chemical compound, drug |
Pierce Phosphatase Inhibitor Mini Tablets |
Thermo Fisher Scientific |
Cat# A32957 |
|
| Chemical compound, drug |
Immobilon Western Chemiluminescent HRP Substrate |
Merck Life Science |
Cat# WBKLS0500 |
|
| Chemical compound, drug |
Rhodamine phalloidin |
Thermo Fisher Scientific |
Cat# R415 |
IF (1:100) |
| Chemical compound, drug |
Wheat Germ Agglutinin, Alexa Fluor 594 Conjugate |
Thermo Fisher Scientific |
Cat# W11262
|
IF (1:500) |
| Chemical compound, drug |
VECTASHIELD Vibrance Antifade Mounting Medium with DAPI |
D.B.A. Italia Srl. |
Cat# H-1800–10 |
|
| Chemical compound, drug |
Collagenase, Type 2 |
Worthington Biochemical Corporation |
Cat# LS004177
|
|
| Chemical compound, drug |
Fura-2, AM, cell permeant |
Thermo Fisher Scientific |
Cat# F1201 |
|
| Chemical compound, drug |
Fluo-4, AM, cell permeant |
Thermo Fisher Scientific |
Cat# F14201
|
|
| Other |
4–20% Criterion TGX Stain Free protein gel,18 well |
Bio-Rad Laboratories |
Cat# 5678094 |
|
| Software, algorithm |
Prism, version 7.0 |
GraphPad Software Inc |
https://www.graphpad.com/scientific-software/prism/RRID:SCR_002798
|
|
| Software, algorithm |
Fiji (ImageJ) (analysis software, version 2.0.0-rc-69/1.52 p) |
National Institute of Health (NIH) |
https://fiji.sc/RRID:SCR_002285
|
SparkMaster plugin used for Ca2+ spark analysis |
| Software, algorithm |
NT Affinity Analysis software, version 2.0.1334 |
NanoTemper Technologies |
N/A |
|
| Software, algorithm |
Ion Wizard, software, version 6.6.11 |
IonOptix B.V. |
N/A |
|
