| Cell line (H. sapiens) |
H9 hESC |
DOI:10.1126/science.282.5391.1145
|
hPSCReg ID: WAe009-A |
|
| Cell line (H. sapiens) |
HeLa control |
other
|
|
Cell line stably expressing Fsp27-GFP from tet-inducible promoter. Obtained from Koini Lim (lab of David Savage). |
| Recombinant DNA reagent |
pCAGEN-SB100X |
DOI:10.1038/nbt.3906
|
|
|
| Recombinant DNA reagent |
pT2-CAG-fGFP |
DOI:10.1038/nbt.3906
|
|
|
| Recombinant DNA reagent |
pT2-CAG-fFusionRed |
DOI: 10.1038/s41593-019-0350-2
|
|
|
| Recombinant DNA reagent |
EGFP-E-Syt1 |
Addgene |
66830 |
|
| Recombinant DNA reagent |
pcDNA3-hL1 |
Addgene |
89411 |
|
| Recombinant DNA reagent |
pT2-CAG-GFP-E-Syt1 |
Generated for this study |
|
|
| Recombinant DNA reagent |
pT2-CAG-hL1CAM-GFP |
Generated for this study |
|
|
| Other |
Gemini X2 HT |
BTX Harvard Apparatus |
45–2041 |
|
| Other |
Petri Dish Platinum Electrode for Tissue Chamber Kit 5 mm |
BTX Harvard Apparatus |
45–0505 |
|
| Other |
Leica VT1000 S |
Leica Biosystems |
|
|
| Commercial assay or kit |
STEMdiff Cerebral Organoid Kit |
STEMCELL Technologies |
08570 |
|
| Other |
Millicell Cell Culture Insert |
Sigma Aldrich |
32011202 |
|
| Other |
ACCUMAX cell dissociation reagent |
Sigma Aldrich |
A7089 |
|
| Antibody |
anti-RPL8 (Rabbit monoclonal) |
Abcam |
ab169538 |
1:200 |
| Antibody |
anti-RPS10 (Rabbit monoclonal) |
Abcam |
ab151550 |
1:200 |
| Antibody |
anti-RPS16 (Rabbit polyclonal) |
Abcam |
ab26159 |
1:200 |
| Antibody |
anti-RPS26 (Rabbit polyclonal) |
Thermo Fisher Scientific |
PA5-65975 |
1:200 |
| Antibody |
anti-S6 ribosomal protein (Mouse monoclonal) |
Cell Signaling Technology |
5548 |
1:100 |
| Antibody |
anti-SMI312 (Mouse monoclonal) |
BioLegend |
837904 |
1:500 |
| Antibody |
anti-MAP2 (Chicken polyclonal) |
Abcam |
ab5392 |
1:500 |
| Commercial assay or kit |
CytoTune EmGFP Sendai Virus Fluorescence Reporter |
Thermo Fisher Scientific |
A16519 |
|
| Chemical compound, drug |
SiR-tubulin |
Spirochrome |
SC002 |
|
| Other |
Millicell Cell Culture Insert, 30 mm diameter, hydrophilic PTFE, 0.4 µm |
Merck Millipore |
PICM0RG50 |
|
| Other |
Disposable biopsy punch, 3.5 mm |
Integra |
33–33 |
|
| Other |
Aspirator tube assemblies for calibrated microcapillary pipettes |
Sigma-Aldrich |
A5177-5EA |
|
| Other |
Capillary Tubes Glass, 50 µl volume, 100 mm length |
Drummond Scientific |
1-000-0500 |
|
| Other |
P-2000 micropipette puller |
Sutter Instrument |
|
|
| Other |
EM grids, gold, 200 mesh, R 2/2 holey carbon |
Quantifoil |
|
|
| Other |
EM grids, gold, 200 mesh, R 3.5/1 holey carbon |
Quantifoil |
|
|
| Software, algorithm |
PEET |
PMID:16917055
|
|
https://bio3d.colorado.edu/PEET/
|
| Software, algorithm |
IMOD |
PMID:8742726
|
|
http://bio3d.colorado.edu/imod/
|
| Software, algorithm |
SerialEM |
PMID:16182563
|
|
http://bio3d.colorado.edu/SerialEM/
|
| Software, algorithm |
ec-CLEM plugin for Icy |
PMID:28139674
|
|
|
| Software, algorithm |
ImageJ |
PMID:22930834
|
|
|
| Chemical compound, drug |
poly-L-ornithine |
Sigma Aldrich |
P4957 |
|
| Chemical compound, drug |
Laminin |
Sigma Aldrich |
L2020 |
|
| Chemical compound, drug |
Fibronectin |
Sigma Aldrich |
F0895 |
|
