Figure 1. Reconstruction of experimental arena and surrounds from the animal’s perspective.

(A) Schematic of experimental arena with olfactory and auditory noise. (B) Schematic of tracking, anatomical and eye camera calibration. Head position and orientation was tracked using seven IR-LEDs (colored circles). Nostrils (red, yellow filled circles), left (blue filled circle), and right (green filled circle) medial canthi were identified and triangulated in calibration images and used to define a common coordinate system (forward, blue arrow, right, green arrow, and up, red arrow), into which the calibrated eye camera location and orientation could also be placed (eye camera vertical, cyan, horizontal, purple, camera optical axis, red). (C) Example left- and right eye camera images with tracked pupil position (white dashed outlines). (D) Rendered digital reconstruction of the laboratory room and (E) experimental arena. (F) Schematic representation of mouse’s left- (blue) and right (green) visual fields, showing also the region of binocular overlap (yellow) and un-seen region (white). (G) Reconstruction of the arena and room from the animal’s left- and right eye perspective, with monocular and binocular regions colored as in (F). (H) Reconstruction of the animal’s view of the prey (cricket - black) in the experiment arena. (I) Representation of left and right eye views of the arena and surrounding objects grayscale-coded by distance from the eye. (J) Rendered animal’s eye views from the left- and right eyes with overlay of arrows representing optic flow during 10 ms of free motion.

Figure 1—figure supplement 1. Generation of mouse eye views during cricket pursuit.

(A) Head pitch (red), roll (blue) and yaw (orange) and associated left (blue) and right (green) horizontal, vertical and torsional eye movements during the 46.2 s, example cricket pursuit sequence shown in C. (Right) Head and eye rotations during the 0.65 s region between i and ii in the cricket pursuit sequence in C. (B) Example (upper rows) head pitch, roll, and horizontal, vertical and torsional, eye rotations during non-pursuit and pursuit sequences (n=1 animal). Lower rows: head and eye rotations during non-pursuit and pursuit sequences from three mice. (C) Mouse (black) and cricket (orange) paths during a 46.2 s segment of a single pursuit sequence for one animal. (D) Photograph of experiment arena and surrounding environment. (E) Digital rendering of the same experiment arena and surrounding environment. (F) Top-down view of the mouse’s left and right monocular and binocular fields of view (mouse’s head would be centered at the intersection point of monocular and binocular fields of view). (G) Cricket (red) position in the rendered left and right eye corneal fields of view of the experiment arena and surrounding environment during the pursuit sequence in C. (H) Trajectory of the projected cricket position in the left and right corneal views, during the pursuit sequence in C.