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. 2021 Oct 22;14:5131–5144. doi: 10.2147/OTT.S322852

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© 2021 Luo et al.

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Effects of 18β-Gly on the cell cycle in A549 cells. (A) The percentage of cell cycle as detected by FCM. (B) Expression levels of G2/M cell cycle-related proteins cyclin B1, CDK1/2, p21, and p27 were examined by Western blotting after treatment with 18β-Gly. (C) A549 cells were treated with NAC and 18β-Gly, and then the percentage of cell cycle was detected by flow cytometry. (D) A549 cells were treated with 18β-Gly and NAC, after which the expression levels of cyclin B1, CDK1/2, p21, and p27 were detected by Western blotting. β-actin was used as the loading control. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001 vs 0 h or the NAC + 18β-Gly group.