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. 2021 Oct 22;14:5131–5144. doi: 10.2147/OTT.S322852

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© 2021 Luo et al.

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Effects of 18β-Gly on cell migration in A549 cells. (A) Transwell migration assay detects cell migration. (B) Cell migration was observed by an inverted microscope. (C) Expression levels of E-cadherin, N-cadherin, vimentin, and SNAI 1 were examined by Western blotting after treatment with 18β-Gly. (D) A549 cells were treated with NAC and 18β-Gly, and then cell migration were observed by an inverted microscope. (E) A549 cells were treated with 18β-Gly and NAC, after which the expression levels of E-cadherin, N-cadherin, vimentin, and SNAI 1 were detected by Western blotting. (F) A549 cells were treated with 18β-Gly and SB203580, after which the expression levels of E-cadherin, N-cadherin, vimentin, and SNAI 1 were detected by Western blotting. (G) A549 cells were treated with 18β-Gly and SP600125, after which the expression levels of E-cadherin, N-cadherin, vimentin, and SNAI 1 were detected by Western blotting. (H) A549 cells were treated with 18β-Gly and FR180204, and the expression levels of E-cadherin, N-cadherin, Vimentin, and SNAI 1 were detected by Western blotting. β-actin was used as the loading control. ns, not significant, *P < 0.05, **P< 0.01, ***P < 0.001 vs 0 h or the NAC + 18β-Gly group.