Figure 10.

The fraction of projections required for a cylindrical sample with no missing wedge to achieve the same quality of reconstruction as the missing wedge datasets for a planar sample (dashed lines) and cylindrical sample (solid lines), for apoferritin (a,b,e,f) and ribosomes (c,d,g,h), with a fixed number of projections and a fixed tilt increment using the FSC average overall (a–d) and in the reciprocal x/z and y/z planes (e–h). For the planar samples, high maximum tilt angles considered to be unphysical due to sample and mechanical limitations are shaded in red.