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Reconstructions of a simulated apoferritin molecule from tilt series with a maximum tilt angle of (a) ± 90°, (b) ± 75°, (c) ± 60° and (d) ± 45°. The features along with the z-axis are progressively blurred as the size of the missing wedge increases. The local 3D FSC for the reconstruction with the known structure at a FSC threshold level of 0.85 for (e) ± 90°, (f) ± 75°, (g) ± 60° and (h) ± 45°.
