Figure 6.

The effect of increasing the maximum tilt angle on the quality of reconstructed data for planar samples (dashed lines) and cylindrical samples (solid lines), for apoferritin (a,b,e,f,i,j) and ribosome samples (c,d,g,h,k,l), with a fixed number of projections and a fixed tilt increment, for sample, thicknesses of 100 nm (a–d), 150 nm (e–h) and 200 nm (i–l). The quality of the reconstruction is assessed by the overall FSC average and by the FSC in high- and low-resolution bins. As the maximum tilt angle increases, the quality of the reconstruction generally improves in each case. For the planar samples, high maximum tilt angles are considered to be unphysical due to sample and mechanical limitations and are shaded in red. In all cases, the cylindrical samples show marginally better reconstruction quality than the planar sample.