Figure 7.

The effect of increasing the maximum tilt angle on the anisotropic FSC average of reconstructed data in the reciprocal x/y, x/z and y/z planes for planar samples (dashed lines) and cylindrical samples (solid lines), for apoferritin (a,b,e,f,i,j) ) and ribosome samples (c,d,g,h,k,l), with a fixed number of projections and a fixed tilt increment, for sample thicknesses of 100 nm (a–d), 150 nm (e–h) and 200 nm (i–l). For the planar samples, high maximum tilt angles are considered to be unphysical due to sample and mechanical limitations and are shaded in red.