Table 2.
Correlation between the measured parameters of Zn supply in the breeders and embryos.
| Item | Breeder stage1 | Embryonic stage2 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Embryonic mortality | Plasma Zn concentration | Erythrocytic ALP activity | Erythrocytic 5′-NT activity | MDA content | MT content | GSH-Px activity | GPx mRNA | MT1 mRNA | BCL2 mRNA | |
| Embryonic mortality | 1.00 | |||||||||
| Plasma Zn concentration | -0.30 | 1.00 | ||||||||
| Erythrocytic ALP activity | -0.42 | 0.71∗∗ | 1.00 | |||||||
| Erythrocytic 5′-NT activity | -0.50 | 0.75∗∗ | 0.61∗ | 1.00 | ||||||
| MDA content | 0.81∗∗ | -0.55∗ | -0.36 | -0.54∗ | 1.00 | |||||
| MT content | -0.50 | 0.26 | 0.63∗∗ | 0.39 | -0.36 | 1.00 | ||||
| GSH-Px activity | -0.45 | 0.80∗∗ | 0.60∗ | 0.68∗∗ | -0.58∗ | 0.22 | 1.00 | |||
| GPx mRNA | -0.39 | 0.63∗∗ | 0.54∗ | 0.69∗∗ | -0.43 | 0.44 | 0.70∗∗ | 1.00 | ||
| MT1 mRNA | -0.40 | 0.75∗∗ | 0.70∗∗ | 0.65∗∗ | -0.49∗ | 0.47∗ | 0.74∗∗ | 0.88∗∗ | 1.00 | |
| BCL2 mRNA | -0.37 | 0.60∗ | 0.49 | 0.68∗∗ | -0.40 | 0.54∗ | 0.67∗∗ | 0.92∗∗ | 0.87∗∗ | 1.00 |
1Embryonic mortality, plasma Zn concentration, erythrocytic ALP, and 5′-NT activity were measured in breeders at week 6 during experimental period. 2The MDA and MT contents, GSH-Px activity, and target gene mRNA expressions were determined in embryonic livers on E29. ALP: alkaline phosphatase; 5′-NT: 5′-nucleotidase; MDA: malondialdehyde; GSH-Px: glutathione peroxidase; GPx: glutathione peroxidase; MT1: metallothionein 1; BCL2: B-cell lymphoma-2.∗P < 0.05; ∗∗P < 0.01.