Skip to main content
. 2021 Oct 19;2021:2999296. doi: 10.1155/2021/2999296

Figure 2.

Figure 2

Effect of CR on apoptosis of renal outer medulla in SD rats with CIN. SD rats were pretreated with CR for 4 weeks. CIN was induced by the intravenous injection of the CM iopromide (1.8 g/kg). The kidney samples were collected 24 h after CM injection. (a1) HE staining of renal outer medulla (scale bar = 50 μm). (a2) Pathological scores. (b1) TUNEL staining of renal outer medulla (scale bar = 50 μm) and (b2) TUNEL-positive cells counted in 10 high-power (400×) fields. (c) Western blot analysis of cleaved caspase 3 (a marker of apoptosis), total caspase-3 expression, and cleaved caspase 3/total caspase 3 ratio. The values are presented as fold-change of control group. (d) Caspase-3 activity detected by ELISA. The values are fold change of the control group; each control sample is given a value of 1. The values are presented as mean ± standard deviation (n = 7, ∗P < 0.05 vs. control, #P < 0.05 vs. CM alone).