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Proteasome inhibition induces HSF HSE binding in the absence of HSF2 activity. Whole cell extracts from control (C), heat-shocked (HS; 42°C for 1 h), hemin-treated (He; 40 μM for 16 h), and MG132-treated (MG; 10 μM for 3 h) mock-transfected K562 cells (vector) or K562 cells overexpressing the HSF2-α (2α-C7) or HSF2-β (2β-D5) isoform were analyzed as for Fig. 1A.
