Table 3.

QuPath and manual counting of positive Bruton tyrosine kinase protein staining, positive Bruton tyrosine kinase protein negative staining, total staining count, the percentage of positive staining, and the relative error (RE) between the two methods.

Core number	Grade	Positive count			Negative count			Total count			Percentage of positive
		QuPath	Manual	RE	QuPath	Manual	RE	QuPath	Manual	RE	QuPath	Manual	RE
118	4	788	790	−0.003	1,740	1,923	−0.095	2,528	2,713	−0.068	31.2	29.1	0.070
101	4	1,118	1,191	−0.061	2,135	2,664	−0.199	3,253	3,855	−0.156	34.4	30.9	0.112
135	2	865	980	−0.117	104	85	0.224	969	1,065	−0.090	89.3	92.0	−0.030
140	2	264	270	−0.022	1,621	1,677	−0.033	1,885	1,947	−0.032	14.0	13.9	0.010
16	3	238	271	−0.122	652	632	0.032	890	903	−0.014	26.7	30.0	−0.109
153	3	642	767	−0.163	2,517	2,957	−0.149	3,159	3,724	−0.152	20.3	20.6	−0.013
14	4	446	478	−0.067	2,528	2,703	−0.065	2,974	3,181	−0.065	15.0	15.0	−0.002
47	4	434	370	0.173	814	897	−0.093	1,248	1,267	−0.015	34.8	29.2	0.191
56	4	801	830	−0.035	1,583	1,695	−0.066	2,384	2,525	−0.056	33.6	32.9	0.022
69	4	943	923	0.022	248	255	−0.027	1,191	1,178	0.011	79.2	78.4	0.011
72	4	694	713	−0.027	1,759	1,676	0.050	2,453	2,389	0.027	28.3	29.8	−0.052
81	4	549	508	0.081	940	1,037	−0.094	1,489	1,545	−0.036	36.9	32.9	0.121
89	4	1,305	1,339	−0.025	1,633	2,115	−0.228	2,938	3,454	−0.149	44.4	38.8	0.146

Red values represent the real difference between the methods. QP, QuPath; Man, manual; RE, relative error.