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. 2021 Oct 19;5(1):e202101228. doi: 10.26508/lsa.202101228

Figure 1. The MLL3/4 complexes associate with PROSER1, a novel proline and serine rich protein, the DNA demethylase TET2 and the glycosyltransferase OGT.

Figure 1.

(A) FLAG-UTX immunoprecipitation (IP) followed by mass spectrometry identifies all known subunits of the MLL3/4 complexes along with PROSER1, a novel serine and proline rich protein, the DNA demethylase TET2 and the glycosyltransferase OGT. SC, spectral counts; TP, peptide counts; abundance = SC × 50 (kD)/protein size (kD). (B) Western blot of FLAG-UTX IP from HEK293 cells confirming interaction of UTX with PROSER1, TET2 and OGT. UTX interacts with the H3K4 methyltransferases MLL3 and MLL4, RBBP5 a core component of the MLL3/4 complexes, PROSER1, TET2, and OGT. HEK293 cells with a FLAG-tag expressing plasmid were used as an IP control. Nuclear extracts were used as input. Actin was used as a loading control for the inputs. (C) Glycerol gradient sedimentation after FLAG-UTX IP reveals co-fractionation of PROSER1 and TET2 with components of the MLL3/4 complexes (red box).