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. 2021 Oct 26;11:21090. doi: 10.1038/s41598-021-00434-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Top panel: A graphical illustration of the pupil stimulation protocol consisting of three stimulation phases. Simultaneous stimulation phase where both the eyes were stimulated; the unilateral stimulation phase where only one eye was stimulated followed by the other; and an alternating stimulation phase where both the eyes got alternatively stimulated. Bottom panel: Example of a pupillogram generated from the recordings of a healthy subject displaying the raw pupil traces from the right eye (in orange) and left eye (in blue). The superimposed traces are the best fit lines based on the double exponential fitting procedure along with the residual errors (red and blue traces displayed at the bottom).