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. 2021 Oct 26;4:1227. doi: 10.1038/s42003-021-02715-9

Fig. 5. Conditional ablation of Bmal1 and Per2 does not affect SCN function.

Fig. 5

a Representative double-plotted actograms illustrating the daily pattern of running-wheel activity of control, Bmal1 heterozygote and knockout female (top), and male (bottom) mice. b Representative double-plotted actograms illustrating the daily pattern of running-wheel activity of control, Per2 heterozygote and knockout female (top) and male (bottom) mice. The vertical marks indicate periods of activity of at least 10-wheel revolutions per 10 min. Each horizontal line plots 48 h, and sequential days are arranged from top to bottom. The empty and gray shaded areas in each actogram illustrate the light and dark phases, respectively. c–v Circadian analysis of locomotor activity of Bmal1 and Per2 control, heterozygote and knockout male and female mice. One way-ANOVA. No significant differences were observed between the different genotypes in any of the parameters analyzed. c–f Amplitude of the locomotor activity rhythm. g–j, time to entrain to a 6-h phase advance. k–n Time to entrain to a 6-h phase delay. o–r Free running period in constant dark (DD). s-v Free running period in constant light (LL). a, b LD, 12:12 h light dark; +6 h, 6-h phase advance; −6h, 6-h phase delay; DD, constant dark; LL, constant light. Bmal1CTR: control, Bmal1HET: Bmal1 heterozygote, Bmal1SKO: Bmal1 knockout. Per2CTR: control, Per2HET: Per2 heterozygote, Per2SKO: Per2 knockout. Bars on the graphs represent the arithmetic mean ± S.E.M.