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. 2021 Oct 26;11:21063. doi: 10.1038/s41598-021-00614-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The characteristics of nicotinine and nicotinine heterosis in different materials. The letters and “*” represent significant differences. Significant differences among the nicotine content at P < 0.05 and P < 0.01 were determined by the Duncan's new multiple range test. (a) The difference of nicotine content in different parent materials. The lowercase letters represents significant difference (P < 0.05); and capital letters represents extremely significant difference (P < 0.01). (b) The difference of nicotine content in tobacco hybrids and their parents. “**” represents extremely significant difference (P < 0.01). (c) Heterosis performance value of nicotine content in hybrid F₁. The OPH, MPH and BPH represents over high-parent heterosis, mid-parent heterosis, and below low-parent heterosis respectively. Figures generated using Microsoft Excel 2016.