Fig. 6. Acvrl1 mRNA expressions and phosphorylation of Smad1/5/8 and STAT3 in the lungs of JAK2^V617F mice in response to chronic hypoxia.

a mRNA expression of Acvrl1 in whole lung extracts (top) or the sorted Ly6G⁺ cells from the lungs (bottom) of WT mice and JAK2^V617F mice exposed to normoxia or hypoxia. The data were normalized to 18 s rRNA levels (n = 5, 6, 5, 6, ^*P = 0.0004, ^†P = 0.0004 for whole lung extracts, n = 5, 5, 6, 6, ^*P = 0.0069, ^†P = 0.0012 [left], <0.0001 [right] for sorted Ly6G⁺ cells). Western blot analysis on the SMAD (b) and STAT (c) pathways in the lungs. Lung extracts from WT mice or JAK2^V617F mice were immunoblotted with the indicated antibodies. The ratios of phosphorylated Smad1/5/8 (p-Smad1/5/8) to total Smad1 (t-Smad1) and phosphorylated-STAT3 (p-STAT3) to total STAT3 (t-STAT3) are shown in the bar graphs. The average value for normoxia-WT mice was set to 1 (b, n = 3 in each group, ^*P = 0.0382, ^†P = 0.0100; c, n = 6 in each group, ^*P = 0.0125 [left], 0.0019 [right], ^†P < 0.0001). GAPDH was used as the loading control. All data are presented as mean ± SEM. ^*P < 0.05 versus the corresponding normoxia-group and ^†P < 0.05 versus the corresponding WT mice by the one-way ANOVA with Tukey post-hoc analysis. Source data are provided as a Source Data file.