TABLE 1.
Comparison of GHRL, GHSR, and MBOAT4 expression changes in post-mortem brain tissue between AUD and controls
| AUD ΔCta | Control ΔCta | AUD 2−ΔΔCtb | Control 2−ΔΔCtb | Statistic | Adjusted P value | |
|---|---|---|---|---|---|---|
| GHRL | ||||||
| HIPP | 12.5 | 12.3 | 0.80 | 1.02 | F(1,17) = 5.311, P = 0.034 Brain pH, PMI, Agec | 0.17 |
| AMY | 10.3 | 10.2 | 0.85 | 0.93 | F(1,19) = 0.846, P = 0.369 | 1 |
| PFC | 12.9 | 13.0 | 1.04 | 0.98 | F (1,20) = 0.354, P = 0.559 | 1 |
| NA | 11.3 | 11.4 | 0.93 | 0.90 | F(1,18) = 0.218, P = 0.646 | 1 |
| VTA | 11.5 | 11.4 | 0.96 | 1.00 | F(1,19) = 3.900, P = 0.063 Pack yearsc | 0.315 |
| GHSR | ||||||
| HIPP | 10.1 | 9.1 | 0.90 | 1.35 | F(1,21) = 1.206, P = 0.284 | 1 |
| AMY | 12.6 | 13.1 | 1.77 | 1.67 | F(1,20) = 0.098, P = 0.758 | 1 |
| PFC | 14.4 | 15.5 | 2.95 | 1.24 | F(1,18) = 4.195, P = 0.055 | 0.275 |
| NA | 10.4 | 9.9 | 1.49 | 1.25 | F(1,18) = 1.126, P = 0.303 Brain pH, PMIc | 1 |
| VTA | 12.9 | 13.1 | 1.18 | 0.91 | F(1,20) = 1.829, P = 0.191 | 0.96 |
| MBOAT4 | ||||||
| HIPP | 14.3 | 14.8 | 0.91 | 1.19 | F(1,19) = 3.036, P = 0.098 | 0.49 |
| AMY | 11.7 | 11.5 | 1.17 | 1.03 | F(1,22) = 1.731, P = 0.202 | 1 |
| PFC | 13.0 | 13.4 | 1.28 | 0.94 | F(1,19) = 3.564, P = 0.074 | 0.37 |
| NA | 12 | 11.6 | 0.84 | 0.97 | F(1,15) = 4.693, P = 0.047 Pack years, BMIc | 0.235 |
| VTA | 15.2 | 15.3 | 0.88 | 0.94 | F(1,14) = 3.502, P = 0.082 Pack yearsc | 0.41 |
Abbreviations: AMY = amygdala, AUD = alcohol use disorder, GHRL = growth hormone receptor ligand, GHSR = growth hormone secretagogue receptor, HIPP = hippocampus, MBOAT4 = membrane-bound o-acyl-transferase 4, NAc = nucleus accumbens, PFC = prefrontal cortex, PMI = post-mortem interval, VTA = ventral tegmental area.
a
ΔCt = Cycle threshold (Ct) of gene of interest (MBOAT4, GHSR, or GHRL)—cycle threshold for GAPDH (housekeeping gene used as endogenous control).
b
2−ΔΔCt calculated based on ΔΔCt = ΔCt AUD −ΔCt controls.
c
Covariates in final model.