FIGURE 5.

NRP1 is one target gene of miR-320d and regulated by NONHSAT114552 and miR-320d. (A). Bioinformatic analysis predicted miR-320d target site in the 3′UTR of NRP1 (B). The picture indicated luciferase reporter plasmid including WT or Mut NRP1 (C). On the basis of luciferase reporter assay, miR-320d significantly decreased luciferase signals of NRP1-WT reporters, but did not affect NRP1-Mut reporters (D). NRP1 mRNA expression was investigated in groups of sh-NONHSAT114552+miR-320d inhibitor, miR-320d inhibitor, sh-NONHSAT114552 or scramble (E). NRP1 Protein expression was measured in groups of sh-NONHSAT114552+miR-320d inhibitor, miR-320d inhibitor, sh-NONHSAT114552 or scramble.