Figure 2. CD56low CD16+ blood NK cells from normal donors as well as psoriasis and AD patients respond to active chemerin.
Total PBMC were tested in transwell chemotaxis, and the migrated cells were stained for CD56 and CD16 to define two circulating NK cell subsets, CD56low CD16+ (A) and CD56hi CD16− (B). The following chemoattractants were used as indicated: CXCL12 (10 nM), CXCL10 (115 nM) and chemerin/SspB (50 pM). Migration to chemotaxis medium served as a negative control (Medium). NK cell subset migration is displayed as the mean of six independent donors in each group ±S.D. Statistically significant differences in the migration to the negative control (chemotaxis medium) vs. various chemoattractants in pairwise comparisons within patient groups was determined by Student’s t-test (P<0.05) and indicated by *.