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. 2021 Oct 26;11:21125. doi: 10.1038/s41598-021-00576-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Site frequency spectra of derived alleles and derived allele proportions. Each population was subsampled to 18 individuals, and fixed sites were excluded. For Tunisian Imazighen, the 18 individuals selected were all of the available in our dataset. For Tunisian non-Imazighen, 18 randomly selected individuals were used. (a) General site frequency spectrum derived alleles for Tunisian populations, together with a panel of surrounding and other worldwide populations. Population names abbreviated as in Fig. 1. (b) General site frequency spectrum of derived alleles for Tunisian populations. (c–f) Site frequency spectra of derived alleles in different GERP RS score categories. Plot title indicates the range of GERP RS scores of the variants included. (g) Proportion of derive deleterious variants classified by frequency-based categories. Variants included are those with GERP RS scores higher than 2. The frequency-based categories are Low-Frequency (LOW), including singletons and doubletons, and Common (COM), including frequencies higher than tripletons. Error bars represent the 95% confidence intervals.