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. 2021 Oct 13;13:748637. doi: 10.3389/fnagi.2021.748637

FIGURE 3.

FIGURE 3

Perioperative use of cefazolin reversed the alterations of BBB permeability and tight junction proteins in the AS group. (A) Hippocampal EB concentration of mice in the four groups. (B) Representative bands and quantitation of ZO-1 and OCLN proteins in the hippocampi (relative to GAPDH). (C) Representative images of DAPI and ZO-1 in the hippocampus for immunofluorescence. (D) Representative images of DAPI and OCLN in the hippocampus for immunofluorescence. Data are shown as mean ± S.E.M. (for EB test, n = 5 in each group; for western blot and immunofluorescence, n = 6 in each group). *P < 0.05 or **P < 0.01. AS, anesthesia and surgery; EB, Evans blue; GAPDH, glyceraldehyde phosphate dehydrogenase; HC, healthy control; NCD, non-POCD; N.S., not significant; OCLN, occludin; POCD, post-operative cognitive dysfunction; ZO-1, zonula occludens 1.