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. 2021 Oct 26;12(11):1003. doi: 10.1038/s41419-021-04282-7

Fig. 6. ER stress inhibitor 4-PBA attenuates ROS generation and mitochondrial dysfunction in CGI-58 knockdown NRCMs.

Fig. 6

A Western blot analysis of GRP78 and CHOP protein levels (n = 4). B JC-1 staining of mitochondrial membrane potential (left), and the ratio of JC-1 in the polarized or aggregate form (red) to the depolarized or monomer form (green) (right, n = 3). C The ROS level in NRCMs was evaluated with MitoSOX (0.5 mM; left), and the quantification of MitoSOX fluorescence from 100 cells of each independent experiment (right, n = 3). D The ATP content in NRCMs (n = 4). E Cardiomyocyte OCR changes examined by Seahorse XF 24 extracellular analyzer. *P < 0.05, **P < 0.01.