Fig. 7. Administration of 4-PBA inhibits cardiac hypertrophic remodeling and dysfunction in CGI-58^cko mice.

A M-model echocardiography of the left ventricle (upper). Measurement of EF% and FS% (lower, n = 8). B Heart size (upper), heart weight, and HW/TL ratio (lower, n = 8). Scale bar 2 mm. C FITC-labeled WGA staining or Masson’s Trichrome staining of the heart sections (upper). Quantification of the relative myocyte cross-sectional area and fibrotic area (lower, n = 5). D DHE or Oil Red O staining of the heart sections (upper), and the quantification of ROS and lipid levels (lower, n = 5). Scale bar 50 μm. E Western blot analysis of GRP78, CHOP, p-IRE, and p-Elf2a protein levels (left), and the quantification of relative protein level (right, n = 4). Data are presented as mean ± SEM, and n represents the number of samples or animals. *P < 0.5, **P < 0.01, ***P < 0.001.