Figure 3.

Histological assessment of Sec24c^+/c-d intercross progeny at P0. (A) H&E stained longitudinal sections of P0 pups from Sec24c^+/c-d intercross taken shortly after birth. A total of 16 animals were analyzed by H&E at this time point. Scale bars = 5 mm. (B) Low and higher magnification views of H&E stained sections through the lung of P0 mice collected shortly after birth. Analysis did not identify gross alterations in the morphology of the respiratory tree, although alveoli from Sec24c^c-d/c-d pups were often uninflated and lined by columnar epithelium compared to the flattened epithelial lining wild type and Sec24c^+/c-d alveoli. Lungs were fixed and sectioned in the context of the whole pup. Scale bars = 500 or 250 microns, as indicated. (C) Longitudinal sections through the vertebral column of Sec24c^c-d/c-d embryos and wild type or Sec24c^+/c-d controls illustrating regions of developing cartilage in forming vertebral bodies by Alcian blue (n = 3 for each genotype, Scale bars = 50 μm) and (D) alizarin red stains (n = 2 for each genotype). (E) Immunohistochemistry for SERT in Sec24c^c-d/c-d and wild type control brain tissues (n = 3 per genotype). Scale bars = 20 μm.