Figure 5.

Analysis of Sec24c^c-d allele splicing. A three-primer RT-PCR reaction using qF1, qR1 and qR3 demonstrates the presence of Sec24c^c-d allele splice variants. Primers qF1 and qR3 (see schematic at the right) detected mRNA transcripts from both the wild type allele (exons 2–3-4–5) and the transcript skipping exon 3 (2-4-5) present in mice carrying the Sec24c^c-d allele, resulting from splicing around the dRMCE insertion (lanes 3–6), as well as mice carrying the Sec24c^- allele, in which exon 3 was also excised (lanes 7–8). Primers qF1 and qR1 detect the mRNA transcript of the Sec24d cDNA insert in Sec24c^+/c-d and Sec24c^c-d/c-d mice (lanes 3–6), and this transcript is absent in wild type littermates (lanes 1–2), or Sec24c^+/− mice (lanes 7–8).