Table 1.
Results of Sec24c+/c-d backcrosses and intercrosses.
| Cross | Genotypes | ||||
|---|---|---|---|---|---|
| (A) Sec24c+/c-d X C57BL/6J | Sec24c+/+ | Sec24c+/c-d | p-value | ||
| Expected | 50% | 50% | |||
| Observed | |||||
| Chimera F1 (n = 38) | 58% (22) | 42% (16) | p > 0.33 | ||
| C57BL/6J N2 (n = 84) | 45% (38) | 55% (46) | p > 0.38 | ||
| Total (n = 122) | 49% (60) | 51% (62) | p > 0.85 | ||
| (B) Sec24c+/c-d X Sec24d+/GT |
Sec24c+/+ Sec24d+/+ |
Sec24c+/c-d Sec24d+/+ |
Sec24c+/+ Sec24d+/GT |
Sec24c+/c-d Sec24d+/GT |
p-value |
|---|---|---|---|---|---|
| Expected | 25% | 25% | 25% | 25% | |
| Observed | |||||
| F1 (n = 99): | 28.3% (28) | 27.2% (27) | 23.2% (23) | 21.2% (21) | > 0.72 |
| (C) Sec24c+/c-d Sec24d+/GT X C57BL/6J |
Sec24c+/+ Sec24d+/+ |
Sec24c+/c-d Sec24d+/+ |
Sec24c+/+ Sec24d+/GT |
Sec24c+/c-d Sec24d+/GT |
|
|---|---|---|---|---|---|
| Expected | 25% | 25% | 25% | 25% | |
| Observed | |||||
| N2 (n = 19): | 36.8% (7) | 15.8% (3) | 21.1% (4) | 26.3% (5) | > 0.60 |
| (D) Sec24c+/c-d intercross | Sec24c+/+ | Sec24c+/c-d | Sec24cc-d/c-d | p-value | |
|---|---|---|---|---|---|
| Expected | 25% | 50% | 25% | ||
| Observed | |||||
| 2 weeks of age (n = 163) | 30% (49) | 70% (114) | 0 | < 1.70 × 10–13 | |
| P0 (n = 97, from 13 litters) | 23% (22) | 50% (49) | 27% (26*) | > 0.68 | |
| All embryonic time points (n = 109) | 28% (31) | 48% (52) | 24% (26) | > 0.78 | |
| E17.5 to E18.5 (n = 80, from 11 litters) | 29% (23) | 41% (41) | 20% (16) | > 0.30 | |
| E15.5 (n = 23, from 3 litters) | 26% (6) | 35% (8) | 39% (9) | > 0.11 | |
| E12.5 to E13.5 (n = 6, from 1 litter) | 33% (2) | 50% (3) | 17% (1) | > 0.63 | |
Distribution of progeny from (A) Sec24c+/c-d backcrosses (B) Sec24c+/c-d x Sec24d+/GT intercrosses, (C) Sec24c+/c-d Sec24d+/GT backcrosses and (D) Sec24c+/c-d intercrosses. Genotypes shown for chimera F1 only include those of chimera/ B6(Cg)-Tyrc-2J/J F1 progeny with black coat color. All Sec24d+/GT mice used in this study were ≥ N18 on C57BL/6J background. For intercross data, p-values are calculated based on “others vs. rescue” genotypes (0.75:0.25). *all Sec24cc-d/c-d mice were dead at P0. The E15.5 time point in (D) contains genotypes for a single Sec24c+/c-d resorbed embryo and two Sec24cc-d/c-d resorbed embryos. All observed numbers are listed in parentheses.