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. 2021 Oct 26;11:21048. doi: 10.1038/s41598-021-99887-5

Figure 2.

Figure 2

Arenaviral NPs transfection constructs. (a,b) Schematic representation of the wild-type (wt) and the mutated (mut) versions of the UGV-1 NP (a) and JUNV NP (b) expressed from a pCAGGS-FLAG or pCAGGS-HA construct, respectively, and used for transfections. Black arrowheads in the putative MTSs (first N-terminal 34 amino acids shown) indicate the positions of the amino acid substitutions of the mut versions compared to the corresponding wt sequences. Both wt and mut UGV-1 NPs are fused in frame with a C-terminal FLAG tag, separated by a linker sequence (a). Both wt and mut JUNV NPs are fused in frame with a C-terminal HA tag, separated by a linker sequence (b).