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. 2021 Oct 26;11:21048. doi: 10.1038/s41598-021-99887-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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IF studies on arenaviral NPs in transfected boid and mammalian cells. Double IF images of Boa constrictor V/4Br cells incubated at 30 °C (a,b) or of monkey Vero E6 cells incubated at 37 °C (c,d), transfected with a construct expressing either wt or mutUGV1-NP-FLAG, UHV1-NP-FLAG, or HISV1-NP-FLAG (a,c), and wt or mutJUNV-NP-HA, or LCMV-NP-HA (b,d) at three dpt. Non-transfected (Mock) cells served as controls. (a,c) The panels from left: FLAG tag in red (secondary antibody: AlexaFluor 594 goat anti-rabbit), mitochondrial marker in green (secondary antibody: AlexaFluor 488 goat anti-mouse), nuclei in blue (DAPI), and a merged image. (b,d) The panels from left: HA tag in red (secondary antibody: AlexaFluor 594 goat anti-rabbit), mitochondrial marker in green (secondary antibody: AlexaFluor 488 goat anti-mouse), nuclei in blue (DAPI), and a merged image.