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. 2021 Oct 13;8:708096. doi: 10.3389/fnut.2021.708096

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Copyright © 2021 Lee, Liao, Lee, Lin, Hsu, Chiou, Young, Lin, Huang and Watanabe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The impact of Lactobacillus plantarum TWK10 on body fat regulation. (A) Representative micro-CT images showing abdominal region composition. (B) Quantification of fat mass at abdominal regions L1 to L5 after 8 weeks of PBS or TWK10 treatment. Epididymal fat pad (EFP) and interscapular brown adipose tissue (BAT) were harvested after 8 weeks of TWK10/PBS administration, and subjected to histological analysis. (C) Representative images of EFP obtained by hematoxylin and eosin (H&E) staining. (D,E) Measurements of EFP wet weight, and the mean cross-section area of EFP adipocytes. (F) Representative images of BAT obtained by H&E staining. (G) Quantification of BAT adipocyte number per high-power-field (HPF). (H) Quantification of BAT brown area after H&E staining. Data are represented as mean ± SD. Statistical differences among groups were analyzed by two-way ANOVA with the post-hoc Tukey-Kramer test and different letters (a, b, c) indicate significant differences, P < 0.05. Non-parametric data were statistically analyzed by Kruskal-Wallis test with Dunn's test.