Table 3.
Lactobacillus plantarum TWK10 enriched SCFA-producing gut bacteria and promoted the production of SCFAs.
| Group | ||||||
|---|---|---|---|---|---|---|
| Y-Control | Y-TWK10 | P-value | A-Control | A-TWK10 | P-value | |
| SCFA (μmol/g cecal content) | ||||||
| Acetate | 28.01 ± 26.91 | 119.90 ± 101.20 | 0.0130 | 44.00 ± 26.70 | 49.08 ± 45.08 | 0.7823 |
| Butyrate | 10.55 ± 7.86 | 46.04 ± 43.53 | 0.0211 | 15.47 ± 12.15 | 23.13 ± 28.11 | 0.4724 |
| Propionate | 3.50 ± 3.06 | 12.18 ± 10.34 | 0.0212 | 6.57 ± 3.85 | 6.64 ± 6.56 | 0.9802 |
| Acetate-producing bacteria (%) | ||||||
| Bacteroides | 3.0305 ± 2.3119 | 2.2954 ± 1.5848 | 0.8148 | 4.0741 ± 2.7387 | 3.2048 ± 1.7169 | 0.5027 |
| Blautia | 0.0957 ± 0.0392 | 0.1562 ± 0.0951 | 0.2766 | 0.4425 ± 0.4702 | 0.5243 ± 0.8987 | 0.8868 |
| Eggerthellaceae | 1.1709 ± 0.5187 | 2.0119 ± 1.1364 | 0.0360 | 0.9072 ± 0.3313 | 1.2209 ± 0.4666 | 0.1308 |
| Fusobacteriaceae | 2.8120 ± 0.7184 | 3.4779 ± 1.3763 | 0.4234 | 1.9417 ± 0.8716 | 2.1964 ± 1.0041 | 0.6556 |
| Prevotellaceae UCG-001 | 6.6855 ± 4.3685 | 5.6362 ± 2.8759 | 0.7430 | 6.0641 ± 3.4124 | 9.1604 ± 4.1470 | 0.1519 |
| Prevotella 9 | 0.0081 ± 0.0044 | 0.0107 ± 0.0042 | 0.1111 | 0.0083 ± 0.0020 | 0.0059 ± 0.0018 | 0.4000 |
| Peptococcaceae | 0.0977 ± 0.0594 | 0.2516 ± 0.1093 | 0.0016 | 0.1171 ± 0.0777 | 0.1707 ± 0.0933 | 0.2060 |
| Ruminococcaceae UCG-004 | 0.0390 ± 0.0255 | 0.1144 ± 0.0631 | 0.0016 | 0.0677 ± 0.0623 | 0.0759 ± 0.0673 | 0.8238 |
| Ruminiclostridium 9 | 0.2943 ± 0.1438 | 0.5067 ± 0.2774 | 0.0745 | 0.2439 ± 0.2088 | 0.4522 ± 0.4725 | 0.4119 |
| Ruminiclostridium 6 | 0.0269 ± 0.0385 | 0.1137 ± 0.1328 | 0.0755 | 0.0989 ± 0.0983 | 0.2440 ± 0.2440 | 0.4396 |
| Streptococcus | 0.1604 ± 0.1029 | 0.2083 ± 0.1344 | 0.5414 | 0.0831 ± 0.0626 | 0.1167 ± 0.0836 | 0.3562 |
| Propionate-producing bacteria (%) | ||||||
| Megamonas | 0.0060 ± 0.0017 | 0.0068 ± 0.0012 | 0.8000 | 0.0055 ± 0.0029 | 0.0047 ± 0.0010 | 0.8000 |
| Phascolarctobacterium | n.d. | 0.0061 ± 0.0021 | – | n.d. | 0.0040 ± 0.0000 | – |
| Butyrate-producing bacteria (%) | ||||||
| Eubacterium spp. | 0.2803 ± 0.3138 | 0.5180 ± 0.2924 | 0.1388 | 0.4041 ± 0.4617 | 0.5456 ± 0.8300 | 0.7103 |
| Faecalibacterium prausnitzii | 0.0086 ± 0.0022 | 0.0141 ± 0.0019 | 0.2000 | 0.0107 ± 0.0051 | 0.0075 ± 0.0000 | – |
| Lachnospiraceae | 19.6801 ± 7.6001 | 28.7954 ± 9.6799 | 0.0464 | 18.4889 ± 12.9901 | 22.1170 ± 13.0379 | 0.4561 |
| Lachnospiraceae UCG-006 | 0.1681 ± 0.1416 | 1.9809 ± 2.0960 | <0.001 | 1.3084 ± 2.3146 | 0.4179 ± 0.2934 | 0.9048 |
| Odoribacter | 1.1620 ± 0.6283 | 1.5569 ± 0.9212 | 0.3704 | 1.2450 ± 0.6954 | 1.2187 ± 0.8926 | 0.8820 |
| Oscillibacter | 0.4131 ± 0.3367 | 0.5855 ± 0.5635 | 0.6058 | 0.3599 ± 0.3216 | 0.4936 ± 0.4787 | 0.8238 |
| Roseburia | 0.1446 ± 0.1095 | 0.3088 ± 0.2865 | 0.1388 | 0.3245 ± 0.2431 | 0.4554 ± 0.6009 | > 0.9999 |
| Ruminococcaceae | 5.1385 ± 2.0922 | 7.4884 ± 2.7297 | 0.0927 | 7.0488 ± 4.4442 | 5.7306 ± 2.9812 | 0.6027 |
Relative abundances of SCFA-associated gut bacteria and the contents of cecal SCFAs in young and aged mice. Data are represented as mean ± SD. Statistical differences between Control and TWK10-administered groups on the basis of the relative abundances of SCFA-producing gut bacteria were analyzed by Mann-Whiney U-test. Statistical significances between the cecal SCFA content of Control and TWK10-administered groups were analyzed by Student unpaired t-test. P < 0.05 represents statistically significant differences. n.d, not detected.