Skip to main content
. 2021 Oct 26;7:313. doi: 10.1038/s41420-021-00661-3

Fig. 3. COL5A1 expression is positively correlated with metastasis ability in GBM cells.

Fig. 3

A qRT-PCR analysis of COL5A1 expression in various GBM cell lines. B Western blot analysis of COL5A1 and tubulin protein expression in the GBM cell panel. C Quantitative results of COL5A1 in the GBM cell panel. D Correlation plot indicating the relationship between COL5A1 mRNA and protein expression levels (Spearman’s rho = 0.733, p = 0.025). E Western blot analysis and quantitative results of COL5A1 knockdown in A-172 and Hs 683 cells. F Western blot analysis and quantitative results of COL5A1 overexpression in LN-229 cells. Tubulin was used as an internal control for protein loading. The significance of the difference was analyzed using the nonparametric Mann–Whitney U test. The AU represents absorbance units.