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. 2021 Oct 13;12:734871. doi: 10.3389/fimmu.2021.734871

Figure 2.

Figure 2

Early cART initiation (4 weeks) restores the loss of CD29hi CD4+ T cells and restores polyfunctionality. (A) Representative gating used to obtain CD4+ CD29 hi T cells used for changes in cytokine secretion at different stages of SIV progression. Briefly, CD4+ T cells were obtained from gated CD3+ T cells/lymphocytes/live cells/singlets/Time vs FSC-A populations. Thereafter, CD29 hi populations were then gated out from CD4+ T cells and the extent of CD95 expression and IFN-γ/TNF-α secretion. Following this, the changes of (B) %CD29hi CD4+ T cells (C) %CD95+ CD29hi CD4+ T cells (D) IFN-γ+ CD29 hi CD4+ T cells (E) TNF-α+ CD29hi CD4+ T cells evaluated at baseline, week 2, week 7 and week 22 post SIV infection. (F) Polyfunctionality profiles of CD29hi CD4+ T cells stimulated with PMA/ionomycin. Included pie charts/bar graphs represent time course changes in CD29hi poly functionality based on IFN-γ, TNF-α and CD95 expression (n = 8). Bar graphs show the median percent cytokine expressing cells. All paired comparisons across the different timepoints were performed using the non-parametric Wilcoxon test. * shows p < 0.05 while ** indicates p < 0.01.