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. 2021 Oct 13;11:759346. doi: 10.3389/fonc.2021.759346

Figure 4.

Figure 4

miR-27a-3p is the regulator of SLC7A11 protein. (A) Predicted the miRNAs targeting SLC7A11 using TargetSan, miRanda, microT, PITA, miRmap, and PicTar databases. (B) Volcano plot indicating the downregulated and upregulated microRNA in NSCLC tissues. (C) Three overlapping miRNAs were screened out including miR-26a-5p, miR-26b-5p, and miR-27a-3p. (D) Measurement of miR-27a-3p expression in the tissues and cell lines of NSCLC. (E) The correlation of SLC7A11 with miR-27a-3p. (F) Determination of the SLC7A11 expression level in miR-27a-3p mimic and anti-miR-27a-3p treated A549 cells, respectively. (G) Prediction of miR-27a-3p’s e binding sites in SLC7A11. (H) Detection of luciferase activity of A549 cells with cotransfection of SLC7A11 (WT) or SLC7A11 (MUT) and miR-27a-3p or anti-miR-27a-3p. **P < 0.01 and ***P < 0.001.