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. 2021 Oct 13;12:754038. doi: 10.3389/fphar.2021.754038

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Copyright © 2021 Ren, Jin, Hu, Chen, Yang, Wu, Zhou, Sun, Gao, Zhang and Tian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Betulin treatment can inhibit the generation of pro-inflammatory mediators. OA chondrocytes were pretreated with betulin (0, 25, 50, and 100 μM) of different concentrations for 2 h, then stimulated by IL-1β (10 ng/ml) for 24 h. (A–B) The protein expression of iNOS and COX-2 was detected by Western blot and quantitatively analyzed. (C–D) The mRNA expression levels of iNOS, COX-2, IL-6, and TNF-α were detected by RT-PCR. (E–F) The generation of NO, PGE2, TNF-α, and IL-6 was detected by Griess reaction and the ELISA method. The data in the figure are represented as the average ±SD, *p < 0.05, **p < 0.01 compared with the control group, ^@@ p < 0.01 compared with the IL-1β group, n = 3.