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. 2021 Oct 13;12:754038. doi: 10.3389/fphar.2021.754038

FIGURE 3.

FIGURE 3

Betulin treatment can inhibit the degradation of extracellular matrix. OA chondrocytes were cultured with betulin (0, 25, 50, and 100 μM) of different concentrations for 2 h, followed by stimulation with or without IL-1β (10 ng/ml) for 24 h. (AB) The protein expression levels of Collagen II, Aggrecan, MMP13, and ADAMTS-5 were detected by Western blot and quantitatively analyzed. (CF) Immunofluorescence staining combined with DAPI staining (scale: 25 μm) was used to detect the fluorescent expression of MMP13.The data in the figure are represented as the mean ± SD, **p < 0.01 compared with the control group, @@ p < 0.01 compared with the IL-1β group, n = 3.