FIG. 1.

Mapping of regions in DRIP205 required for VDR interaction. (A) Schematic representation of the various DRIP205 fragments fused to GST and used as baits in in vitro pull-down assays. The two potential nuclear receptor interaction motifs (NR1 and NR2) are indicated. (B) GST pull-down assay using GST-DRIP205 deletion mutants and [³⁵S]VDR wild type (top) or [³⁵S]VDR AF-2 deletion mutant (bottom). All incubations were carried out in the presence of 10⁻⁶ M 1,25(OH)₂D₃.