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. 2021 Oct 13;8:725275. doi: 10.3389/fmolb.2021.725275

FIGURE 2.

FIGURE 2

Epithelial-mesenchymal transition (EMT) occurred after irradiation (IR) and is mediated by PIEZO1 signaling. (A) Immunohistochemical analyses found TGF-β1, α-SMA, CTGF, and Fibronectin expression was increased 48 h after IR. (B,C) Western blotting results of rat lung tissues (B) and RLE-6TN cells (C) showed that the expression of EMT-related markers N-cadherin, Vimentin, TGF-β1, α-SMA, CTGF, and Fibronectin were up-regulated and E-cadherin was down-regulated compared to those of the control group 48 h after IR. (D) PIEZO1 mRNA and protein expression were successful inhibited by specific siRNA to PIEZO1 (si-PIEZO1) compared with blank and negative control (NC). (E) Representative Western blotting images and histograms showing that IR-induced EMT-related protein expression changes could be partly reversed by PIEZO1 knockdown. Note: The Western blotting images have been subjected to cropping and stitching inresponse to the reviewers’ comments. The original figures can be seen in the supplemental materials. Data are presented with the means ± SEMs (n = 3). *, p < 0.05; and **, p < 0.01.