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. 2021 Oct 13;8:725275. doi: 10.3389/fmolb.2021.725275

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Copyright © 2021 Huang, Zhang, Guo, Lu, Wang, Cheng, Dong, Lyu, Li and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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siRNA-resistant C/EBPβ plasmid transfection reverse the enhanced PIEZO1 expression by C/EBPβ small interfering RNA (si-C/EBPβ). (A) qPCR results showed that the changes of PIEZO1 and C/EBPβ mRNA levels after transfection with siRNA-resistant C/EBPβ plasmids (OE). (B) Representative Western blotting images and histograms showed that the expression changes of C/EBPβ and PIEZO1 protein after transfection with siRNA-resistant C/EBPβ plasmids. Data are presented with the means ± SEMs (n = 3). **, p < 0.01 vs control; ^##, p < 0.01 vs. si-NC (negative control of siRNA); ^$$, p < 0.01 vs OE-NC (negative control of siRNA-resistant C/EBPβ plasmid); ^%%, p < 0.01 vs si-C/EBPβ; ^&&, p < 0.01 vs. OE-C/EBPβ.