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The confocal microscopy of the Jurkat and Jurkat PD-1 modified by CAR19 or CAR19-T2A-aPD-1-PEBL constructs. Jurkat and Jurkat PD-1 cells were transduced by CAR19 or CAR19-T2A-aPD-1-PEBL lentiviruses and sorted by FACS. The cells were fixed and stained with Hoechst, anti-PD-1 antibodies and CAR19 detection agent.
