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. 2021 Oct 13;9:735888. doi: 10.3389/fcell.2021.735888

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Copyright © 2021 Torres, Barrera, Varas-Godoy, Arancibia and Inestrosa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Wnt ligand expression levels in HT-22 cells. (A) Comparison of western blot analysis of Wnt3a, Wnt5a, Wnt7a, and GPR177 protein content in cell lysate and P100 fraction. Each lane was loaded with 30 μg protein. (B) Summary densitometric measurement of band intensity showing enrichment (fold) of each protein in the P100 pellet relative to a corresponding lysate. Wnt3a, 0.4 ± 0.19 (n = 5); Wnt5a, 8.70 ± 4.14 (n = 7); Wnt7a, 0.22 ± 0.21 (n = 4; four independent cell culture preparations); GPR177 3.30 ± 1.93 (n = 3; independent cell culture preparations).